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247 pcmv pe2 p2a gfp  (Addgene inc)


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    Structured Review

    Addgene inc 247 pcmv pe2 p2a gfp
    247 Pcmv Pe2 P2a Gfp, supplied by Addgene inc, used in various techniques. Bioz Stars score: 95/100, based on 51 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pcmv+pe2+p2a+gfp+plasmid/pCMV-PE2-P2A-GFP+(Plasmid+%23132776)/10__1158_slash_0008___5472__can___25___2893-94-9-11
    Average 95 stars, based on 51 article reviews
    247 pcmv pe2 p2a gfp - by Bioz Stars, 2026-10
    95/100 stars

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    Related Articles

    Plasmid Preparation:

    Article Title: ATF4-dependent fructolysis fuels growth of glioblastoma multiforme.
    Article Snippet: .. Briefly, GBM cells seeded onto a six-well plate at confluency of ~60% were co-transfected with pCMV-PE2-P2A-GFP plasmid (#132776, Addgene) and pU6-pegRNA-GG-acceptor plasmid containing the desired pegRNA. ..

    Article Title: Precise genomic deletions using paired prime editing
    Article Snippet: .. We designed pairs of pegRNAs specifying 24-, 91- and 546-bp deletions within the eGFP coding region of the pCMV-PE2-P2A-GFP plasmid (Addgene #132776) ( ). ..

    Article Title: DNA sequence quantitatively encodes CTCF-binding affinity at genome scale
    Article Snippet: .. For transfection, 10 μg of the pCMV-PE2-P2A-GFP plasmid (Addgene; #132776), 10 μg of pegRNA plasmid, and 5 μg of ngRNA plasmid were mixed and delivered into cells using a Neon transfection system (Thermo Fisher Scientific) under the following conditions: 1,400 V, 10 ms, and three pulses. ..

    Article Title: Precise genomic deletions using paired prime editing.
    Article Snippet: .. We designed pairs of pegRNAs specifying 24-, 91- and 546-bp deletions within the eGFP coding region of the pCMV-PE2-P2A-GFP plasmid (Addgene, catalog no. 132776; Fig. 1d). ..

    Article Title: ATF4-dependent fructolysis fuels growth of glioblastoma multiforme
    Article Snippet: .. Briefly, GBM cells seeded onto a six-well plate at confluency of ~60% were co-transfected with pCMV-PE2-P2A-GFP plasmid (#132776, Addgene) and pU6-pegRNA-GG-acceptor plasmid containing the desired pegRNA. ..

    Transfection:

    Article Title: DNA sequence quantitatively encodes CTCF-binding affinity at genome scale
    Article Snippet: .. For transfection, 10 μg of the pCMV-PE2-P2A-GFP plasmid (Addgene; #132776), 10 μg of pegRNA plasmid, and 5 μg of ngRNA plasmid were mixed and delivered into cells using a Neon transfection system (Thermo Fisher Scientific) under the following conditions: 1,400 V, 10 ms, and three pulses. ..



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